Knowledge and awareness of infection control practices among nursing professionals: A cross-sectional survey
Keywords:
Infection control; standard precautions; knowledge; compliance; nursing; cross‑sectional studyAbstract
Background: Infection prevention and control (IPC) is critical for patient safety, yet a gap often exists between nurses’ knowledge and clinical practice. This study aimed to assess infection control knowledge and self‑reported compliance among nurses and to examine associated factors.
Methods: A cross‑sectional study was conducted among 188 nurses at Al Habib Hospital, Saudi Arabia. Data were collected using a validated questionnaire covering sociodemographics, knowledge (20 items, score 0–20), and self‑reported compliance (20 items, 0–4 Likert scale, total 0–80). Descriptive statistics, Mann‑Whitney U, Kruskal‑Wallis H, and Spearman’s correlation were used.
Results: The majority were female (92.0%), held a bachelor’s degree (93.1%), and worked in an adult ICU (64.4%). Mean knowledge score was 14.93±1.75 (74.7%), and mean compliance score was 76.61±6.19 (95.8%). No significant correlation was found between knowledge and compliance (r=0.049, p=0.504). Significant differences in compliance were observed by education level (Bachelor's median 79 vs. Master’s 74, p=0.004) and working unit (emergency lowest median 71, p=0.014). Professional rank was significantly associated with knowledge (registered nurses median 15 vs. senior/head nurses 14, p=0.009). Participation in IPC training was not associated with either outcome.
Conclusion: Nurses demonstrated moderate knowledge but very high self‑reported compliance, with no correlation between the two. Education level and working unit significantly influenced compliance, while training participation did not. Interventions should address unit‑specific barriers and move beyond passive education to improve IPC adherence.
References
1. World Health Organization. Global report on infection prevention and control: executive summary. World Health Organization; 2022 May 26.
2. Haque M, Sartelli M, McKimm J, Bakar MA. Health care-associated infections–an overview. Infect Drug Resist. 2018 Nov 11:2321-33. doi: 10.2147/IDR.S177247
3. Schreiber PW, Sax H, Wolfensberger A, Clack L, Kuster SP. The preventable proportion of healthcare-associated infections 2005–2016: systematic review and meta-analysis. Infect Control Hosp Epidemiol. 2018 Nov;39(11):1277-95. doi: 10.1017/ice.2018.183
4. Sharma M, Bachani R. Knowledge, attitude, practice, and perceived barriers for the compliance of standard precautions among medical and nursing students in central India. Int J Environ Res Public Health. 2023 Apr 12;20(8):5487. doi: 10.3390/ijerph20085487
5. Porto JS, Marziale MH. Reasons and consequences of low adherence to standard precautions by the nursing team. Rev Gaucha Enferm. 2016;37:e57395. doi: 10.1590/1983-1447.2016.02.57395
6. Russell D, Dowding DW, McDonald MV, Adams V, Rosati RJ, Larson EL, et al. Factors for compliance with infection control practices in home healthcare: findings from a survey of nurses' knowledge and attitudes toward infection control. Am J Infect Control. 2018 Nov 1;46(11):1211-7. doi: 10.1016/j.ajic.2018.05.005
7. McDonald L. Florence Nightingale, nursing, and health care today. Springer Publishing Company; 2017 Dec 15.
8. Alhumaid S, Al Mutair A, Al Alawi Z, Alsuliman M, Ahmed GY, Rabaan AA, et al. Knowledge of infection prevention and control among healthcare workers and factors influencing compliance: a systematic review. Antimicrob Resist Infect Control. 2021 Jun 3;10(1):86. doi: 10.1186/s13756-021-00957-0
9. Labrague LJ, McEnroe-Petitte DM, van de Mortel T, Nasirudeen AMA. A systematic review on hand hygiene knowledge and compliance in student nurses. Int Nurs Rev. 2018 Sep;65(3):336-48. doi: 10.1111/inr.12410
10. Houghton C, Meskell P, Delaney H, Smalle M, Glenton C, Booth A, et al. Barriers and facilitators to healthcare workers' adherence with infection prevention and control guidelines for respiratory infectious diseases: a rapid qualitative evidence synthesis. Emergencias. 2021 Feb;33(1):62-4. PMID: 33496401.
11. Padigos J, Reid S, Kirby E, Broom J. Knowledge, perceptions and experiences of nurses in antimicrobial optimization or stewardship in the intensive care unit. J Hosp Infect. 2021 Mar;109:10-28. doi: 10.1016/j.jhin.2020.12.003
12. Hessels AJ, Genovese-Schek V, Agarwal M, Wurmser T, Larson EL. Relationship between patient safety climate and adherence to standard precautions. Am J Infect Control. 2016 Oct 1;44(10):1128-32. doi: 10.1016/j.ajic.2016.03.060
13. Adams V, Song J, Shang J, McDonald M, Dowding D, Ojo M, et al. Infection prevention and control practices in the home environment: Examining enablers and barriers to adherence among home health care nurses. Am J Infect Control. 2021 Jun;49(6):721-6. doi: 10.1016/j.ajic.2020.10.021
14. Lwanga SK, Lemeshow S, World Health Organization. Sample size determination in health studies: a practical manual. World Health Organization; 1991.
15. Al-Faouri I, Okour SH, Alakour NA, Alrabadi N. Knowledge and compliance with standard precautions among registered nurses: A cross-sectional study. Ann Med Surg (Lond). 2021 Jan 29;62:419-24. doi: 10.1016/j.amsu.2021.01.058
16. Gupta R, Burri RR, Bhagavatula S, Patra A, Chandok R, Altaf I, et al. Mapping Nurses' Knowledge, Attitudes, and Practices to Guide Competency-Based Infection Control Training. Cureus. 2025 Oct 23;17(10):e95258. doi: 10.7759/cureus.95258
17. Mhalees EF. Knowledge, Attitude, Practice, and Perceptions among nurses in governmental hospitals towards standard isolation precautions in the West Bank (Doctoral dissertation, Al-Quds University).
18. Batran R, Ayed A, Batran A, Ejheisheh MA, Alassoud B, Hayek MF, et al. Determinants of Nurses' Compliance with Infection Prevention and Control Practices in Critical Care Units. SAGE Open Nurs. 2025 Apr 30;11:23779608251339193. doi: 10.1177/23779608251339193
19. Bawaqneh KA, Ayed A, Salameh B. Nurses' Knowledge, Attitude, Practice, and Perceived Barriers of Infection Control Measures in the Intensive Care Units at Northwest Bank Hospitals. Crit Care Nurs Q. 2025 Apr-Jun 01;48(2):160-71. doi: 10.1097/CNQ.0000000000000538
20. Chowdhury F, Sayem MA, Hossain MA, Salam MA, Khan AA, Nurunnabi AS, et al. Knowledge, attitudes and perceptions of healthcare workers on antimicrobial stewardship and antibiotic use: a nationwide multi-center study in Bangladesh. Discover Public Health. 2025 Nov 24;22(1):741. doi: 10.1186/s12982-025-01107-2
Downloads
Published
Issue
Section
License
Copyright (c) 2026 Ahmad A. Alharbi (Author)

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Transfer of Copyright and Permission to Reproduce Parts of Published Papers.
Authors retain the copyright for their published work. No formal permission will be required to reproduce parts (tables or illustrations) of published papers, provided the source is quoted appropriately and reproduction has no commercial intent. Reproductions with commercial intent will require written permission and payment of royalties.

